1. Turn off Aquarium Lights and be sure to dim down or shut off lighting in room where the box will be opened. Fish are not able to close their eyes so adjusting to lighting can be just as or more stressful than any other kind of acclimation.
2. Have a bucket prepared next to your system. If you’re using a “Reef Pro” acclimation kit, dump contents of packet included in kit into bucket. Carefully open bags (you may need bags near end of acclimating process) of specimens and empty contents including all water into the buckets. Be very careful not to expose sensitive inverts, such as starfish, to air.
3. If there is a small amount of water in your bag, you may need to tilt your bucket at a 45-degree angle to make sure the specimen is completely submerged.
4. Set up Drip Acclimation kit. You will need one kit for each bucket used. The rigid tubing will need to be over the trim of the aquarium with an end in the water. The valve included will be to regulate the flow of the water going into the bucket.
5. Next you will begin a siphon by sucking on the end of the airline and adjusting the drip to about 1 drip a second. After 30 minutes have passed you may raise the amount of drips to about 2-4 drops a second.
6. When the amount of water in the bucket doubles, be sure to empty it by half. Then simply wait for it to double once again. Approximately 1 hour.
7. At this point the specimens are almost ready. Now you will place the specimens in a bag and float bag in aquarium for about 15-20 minutes to acclimate specimens to tank temperature.
8. Once time has elapsed, specimens are ready to be released into tank. Be sure to discard all water from the acclimation process. The water has been fouled in this process and you would not want to add any into your system.
9. Remember to never touch fish by hand, as it may remove some of their protective slime and harm the specimen in the process, stressing it and making its survival chances lower.